Journal: Cell Death Discovery
Article Title: Neural stem cells derived from α-synuclein-knockdown iPS cells alleviate Parkinson’s disease
doi: 10.1038/s41420-024-02176-z
Figure Lengend Snippet: A Schematic illustration of the behavior analysis protocol. At the age of 5 months, six training sessions were conducted for SNCA A53T transgenic mice over 2 weeks. Following the last training, mice were transplanted with NSCs or NSC-shSNCA cells. In a control group (mock), mice were injected with normal saline. NSCs and NSC-shSNCA cells were assessed for their therapeutic effects by B beam walking (mock vs NSC: week 15 to week 13, P < 0.001; mock vs NSC-shSNCA: week 7, P < 0.01, week 5 and 9 to 15, P < 0.001; NSC vs NSC-shSNCA: week 13, P < 0.05, week 15, P < 0.001), C rotarod (mock vs NSC: week 5 to week 15, P < 0.001; mock vs NSC-shSNCA: week 5 to week 15, P < 0.001; NSC vs NSC-shSNCA: NS), D total traveled distance (mock vs NSC: week 13, P < 0.05; mock vs NSC-shSNCA: NS; NSC vs NSC-shSNCA: NS), E locomotion time (mock vs NSC: week 13, P < 0.01, week 15, P < 0.05; mock vs NSC-shSNCA: week 15, P < 0.01; NSC vs NSC-shSNCA: NS), and F rest time (mock vs NSC: week 13 and 15, P < 0.01; mock vs NSC-shSNCA: week 15, P < 0.01; NSC vs NSC-shSNCA: NS). The behavioral assessment was conducted 1 day (week 0) before transplantation and every 7 days thereafter for 22 weeks. Data are presented as mean ± SEM values ( B – F ). Behavioral data were compared by two-way analysis of variance (ANOVA) for time and treatment effects followed by a post hoc Bonferroni test (corrected for multiple comparisons). G Overall survival curves for transplanted mice in the behavior tests. Survival analysis was done using the Kaplan-Meier estimator and the log-rank test for group comparison. Variables with a significant P -value in the univariate analysis were exposed to a multivariate analysis using Cox regression proportional hazard model. GraphPad Prism 5.01 (San Diego, CA, USA) was used for analysis with a significance level of P < 0.05. NS stands for not statistically significant.
Article Snippet: Variables with a significant P -value in the univariate analysis were exposed to a multivariate analysis using Cox regression proportional hazard model. GraphPad Prism 5.01 (San Diego, CA, USA) was used for analysis with a significance level of P < 0.05.
Techniques: Transgenic Assay, Control, Injection, Saline, Transplantation Assay, Comparison
